Using a water chiller, air stones, or an o2 emitter the goal is the same, increase DO. The temperature of the solution plays no other role other than to dictate the saturation point. Bacterial growth would not be inhibited until the water temperature was 3.9 C or 39F, and even at that temperature it is only slowed not stopped. It really is all about oxygen in the root zone. 7-8 ppm is all that is required and can easily be achieved with an adequately sized pump and airstones at temperatures of 70-75 F. I run slightly warmer and use a pump designed for 8 buckets in a 6 bucket system and my plant growth is vigorous.