PigSquishy
New member
Which is a great idea... but that is why I am asking about it here... because I have been trying to find these so called "experts" but like yourself there is so many diversions of this... it would be like saying you have a Ph.D and thus you are a Doctor... a doctor of what, medicine? If so what kind of medicine... because this field is so diverse, and again like you stated earlier yourself, what plants am I going to be working with? Again it gets so diverse... so hey how about I go to a Cannabis Forum, ask if anyone knows anything about this topic, learn all I can from there.. learn how to do it on cannabis where others have tried and maybe done it and have some answers... learn all I did about it and by doing it just like I did with all the different types of cloning over the years... then find out what more I can do with it as I get better at it with time and practice. So I came here and ask what I assumed to be a basic question trying to get to the bottom of what I assumed was fairly straight forward answer...
If I bought a DIY Home Kit, do I just cut up a leaf inside the laminar flow hood keeping everything sterile, find out if I then grow the explants in there, another place, etc... and get a ballpark idea as to the cost of this and what all it is good for. I've heard mother plants can be kept small as explants for years, then grown to full sized mothers if you want to regrow that strain at a later point and keep it in stasis if you don't need it. How hard is it to keep it this way? I came asking with the assumption it couldn't be that hard, and hoping to find out if the different mediums were a preference like strain types, different folks just like working with one over another for their own reasonings. Didn't think this was over the top learning... I keep coming back to a sentence in the book:
The Cannabis Breeders Bible by Greg Green, Chapter 8 Tissue Culture, pg 92, paragraph-1 "TISSUE CULTURE IS A RELATIVELY NEW CLONING METHOD involving a certain degree of lab work. However this is twenty-first century and this kind of lab equipment can cost as little as $40. What follows is a basic rundown of what tissue culture is and how it works."
Now I know how to work in sterile conditions and how to work with medical tools and my basics of lab, I am very skilled with my hands and very knowledgeable, I have the dedication and the willpower to spend hours learning, studying and mastering this skill set; thus I came in search of answers. If I go accordingly to the first kit I researched over at GrowersHouse, a place I know I can trust because they won't carry a product unless the company gives them one for free and then picks the best staff member to give it a try and see if it works and is something they feel comfortable selling customers knowing it will have a high success rate. I know the common household method involves something to do with working within steam to keep the air around the tiny work area sterile. I know what it means to do this kind of work and I know full well to skip that and buy or DIY a laminar flow hood. So yeah I have the basics figured out and want to make sure I increase my success rate by getting set up correctly and understanding if this is really something worth all the time, money, energy of mastering this.
If I bought a DIY Home Kit, do I just cut up a leaf inside the laminar flow hood keeping everything sterile, find out if I then grow the explants in there, another place, etc... and get a ballpark idea as to the cost of this and what all it is good for. I've heard mother plants can be kept small as explants for years, then grown to full sized mothers if you want to regrow that strain at a later point and keep it in stasis if you don't need it. How hard is it to keep it this way? I came asking with the assumption it couldn't be that hard, and hoping to find out if the different mediums were a preference like strain types, different folks just like working with one over another for their own reasonings. Didn't think this was over the top learning... I keep coming back to a sentence in the book:
The Cannabis Breeders Bible by Greg Green, Chapter 8 Tissue Culture, pg 92, paragraph-1 "TISSUE CULTURE IS A RELATIVELY NEW CLONING METHOD involving a certain degree of lab work. However this is twenty-first century and this kind of lab equipment can cost as little as $40. What follows is a basic rundown of what tissue culture is and how it works."
Now I know how to work in sterile conditions and how to work with medical tools and my basics of lab, I am very skilled with my hands and very knowledgeable, I have the dedication and the willpower to spend hours learning, studying and mastering this skill set; thus I came in search of answers. If I go accordingly to the first kit I researched over at GrowersHouse, a place I know I can trust because they won't carry a product unless the company gives them one for free and then picks the best staff member to give it a try and see if it works and is something they feel comfortable selling customers knowing it will have a high success rate. I know the common household method involves something to do with working within steam to keep the air around the tiny work area sterile. I know what it means to do this kind of work and I know full well to skip that and buy or DIY a laminar flow hood. So yeah I have the basics figured out and want to make sure I increase my success rate by getting set up correctly and understanding if this is really something worth all the time, money, energy of mastering this.